製品の概要

  • 製品名Anti-XLF antibody [1F3]
    XLF 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1F3] to XLF
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human XLF.

  • ポジティブ・コントロール
    • HEK293T cellstransfected with pCMV6-ENTRY XLF cDNA, Human breast, Human Kidney, Adenocarcinoma of Human ovary, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate and Carcinoma of Human bladder tissues, COS7 cells transiently transfected with XLF
  • 特記事項Dilute in PBS (pH7.3) before use.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名1F3
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 33 kDa.
IHC-P 1/150.
ICC/IF 1/100.

ターゲット情報

  • 機能DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
  • 組織特異性Ubiquitously expressed.
  • 関連疾患Defects in NHEJ1 are the cause of severe combined immunodeficiency due to NHEJ1 deficiency (NHEJ1-SCID) [MIM:611291]; also known as autosomal recessive T cell-negative, B cell-negative, NK cell-positive, severe combined immunodeficiency with microcephaly, growth retardation and sensitivity to ionizing radiation or NHEJ1 syndrome. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. NHEJ1-SCID is characterized by a profound T- and B-lymphocytopenia associated with increased cellular sensitivity to ionizing radiation, microcephaly and growth retardation. Some patients may manifest SCID with sensitivity to ionizing radiation without microcephaly and mild growth retardation, probably due to hypomorphic NHEJ1 mutations.
    Note=A chromosomal aberration involving NHEJ1 is found in a patient with polymicrogyria. Translocation t(2;7)(q35;p22).
  • 配列類似性Belongs to the XLF family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cernunnos antibody
    • FLJ12610 antibody
    • NHEJ 1 antibody
    • Nhej1 antibody
    • NHEJ1, S. cerevisiae, homolog of antibody
    • NHEJ1_HUMAN antibody
    • Non homologous end joining factor 1 antibody
    • Non-homologous end-joining factor 1 antibody
    • Nonhomologous end joining factor 1 antibody
    • OTTHUMP00000164168 antibody
    • OTTHUMP00000206275 antibody
    • OTTHUMP00000206279 antibody
    • Protein cernunnos antibody
    • XLF antibody
    • XRCC4 like factor antibody
    • XRCC4-like factor antibody
    see all

Anti-XLF antibody [1F3] 画像



  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: XLF knockout HAP1 cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green – ab119375 observed at 38 kDa. Red - loading control, ab129002, observed at 124 kDa.

    ab119375 was shown to specifically react with XLF when XLF knockout samples were used. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab119375 and ab129002 (loading control to Vinculin) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-XLF antibody [1F3] (ab119375) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY XLF cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203393)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human prostate tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using ab119375 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF using ab119375 at a dilution of 1/100.

Anti-XLF antibody [1F3] (ab119375) 使用論文

ab119375 has not yet been referenced specifically in any publications.

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