製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to XIRP1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 1337-1367 of Human XIRP1 (NP_919269.2).

  • ポジティブ・コントロール
    • NCI-H460 cell line lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab116138 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 199 kDa.

ターゲット情報

  • 機能
    Protects actin filaments from depolymerization.
  • 組織特異性
    Isoform A, isoform B and isoform C are expressed in heart.
  • 配列類似性
    Belongs to the Xin family.
    Contains 17 Xin repeats.
  • ドメイン
    Xin repeats bind F-actin.
  • 細胞内局在
    Cell junction. Colocalizes with actin stress fibers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI415219 antibody
    • Cardiomyopathy-associated protein 1 antibody
    • CMYA1 antibody
    • DKFZp451D042 antibody
    • DKFZp779C1255 antibody
    • DKFZp779C1947 antibody
    • MGC144145 antibody
    • MGC144146 antibody
    • Xin actin-binding repeat-containing protein 1 antibody
    • XIN antibody
    • Xirp1 antibody
    • XIRP1_HUMAN antibody
    see all

画像

  • Anti-XIRP1 antibody (ab116138) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 199 kDa

プロトコール

参考文献

ab116138 has not yet been referenced specifically in any publications.

レビューと Q&A

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Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (heart)
Specification
heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jun 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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