Anti-xCT 抗体 (ab37185)
Key features and details
- Rabbit polyclonal to xCT
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-xCT antibody
xCT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to xCT -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human xCT aa 1-50 (N terminal). The exact sequence is proprietary.
Sequence:MVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQLKRKVTLLRGV
Database link: Q9UPY5 -
ポジティブ・コントロール
- ICC/IF: HepG2 cells. IHC-P: Human small intestine tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Affinity purified antibody. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37185の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/1000.
|
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. -
配列類似性
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23657 Human
- Omim: 607933 Human
- SwissProt: Q9UPY5 Human
- Unigene: 390594 Human
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製品の状態
xCT has a predicted molecular weight of 55 kDa; however it has a high number of hydrophobic residues which may affect the migration of the protein in SDS-PAGE. Endogenous monomeric xCT is expected to migrate at ~35 kDa and modified-xCT is expected to migrate at ~55 kDa (PMID: 17035536) -
別名
- Amino acid transport system xc xCT antibody antibody
- Amino acid transport system xc- antibody
- Calcium channel blocker resistance protein CCBR1 antibody
see all
画像
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Imunocytochemistry/ Immunofluorescence analysis of HepG2 cells labeling xCT with ab37185 followed by Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red) respectively.
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Immunohistochemical analysis of human small intestine labeling xCT with ab37185. Staining is observed in the absorptive epithelia of small intestinal villi.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (87)
ab37185 は 87 報の論文で使用されています。
- Müller F et al. Elevated FSP1 protects KRAS-mutated cells from ferroptosis during tumor initiation. Cell Death Differ 30:442-456 (2023). PubMed: 36443441
- Wei TT et al. Interferon-γ induces retinal pigment epithelial cell Ferroptosis by a JAK1-2/STAT1/SLC7A11 signaling pathway in Age-related Macular Degeneration. FEBS J 289:1968-1983 (2022). PubMed: 34741776
- Sendo K et al. Impact of the glutathione synthesis pathway on sulfasalazine-treated endometrial cancer. Oncotarget 13:224-236 (2022). PubMed: 35106124
- Huang S et al. Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis. Cell Mol Gastroenterol Hepatol 13:1649-1672 (2022). PubMed: 35202887
- Zhang R et al. Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis. Bioact Mater 13:23-36 (2022). PubMed: 35224289