製品の概要

  • 製品名Anti-xCT antibody
    xCT 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to xCT
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    KGQTQNFKDAFSGRD

    by a Cysteine residue linker, corresponding to internal sequence amino acids 215-229 of Human xCT.

  • ポジティブ・コントロール
    • Human Kidney and Placenta tissues; Human Spleen lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/128000.
WB Use a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

ターゲット情報

  • 機能Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • 配列類似性Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Anti-xCT antibody 画像

  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Kidney tissue by Immunohistochemistry.
  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.
  • Anti-xCT antibody (ab111822) at 0.02 µg/ml (for one hour) + Human Spleen lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa

Anti-xCT antibody (ab111822) 使用論文

ab111822 has not yet been referenced specifically in any publications.

Product Wall

We have added product ab178004, xCT peptide, as a blocking peptide to be used with ab111822. Here is a link: http://www.abcam.com/xct-peptide-ab178004.html.

Application Flow Cytometry
Sample Human Cell (liver hepatocellular cells HepG2)
Specification liver hepatocellular cells HepG2
Preparation Cell harvesting/tissue preparation method: trypsinized and resuspended in PBS+1% serum+0.1% NaN3
Sample buffer: PBS+1% serum+0.1% NaN3
Fixation did not fix
Permeabilization No
Gating Strategy ungated cell population
Username

Yuzhe

Verified customer

投稿 Sep 05 2012

Thank you for contacting us. You are correct, ab111822 is raised against an extracellular region of xCT. As such you should not have to permeablize your cells to use this product. I hope that this information is helpful. Please let me know if y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"