製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to XBP1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Dog, Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide:

    NHSWEDTFANELFPQ

    by a Cysteine residue linker, corresponding to C terminal amino acids 358-372 of Human XBP1 isoform 2

  • ポジティブ・コントロール
    • Human breast tissue; HeLa nuclear lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
ELISA 1/4000.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • 関連疾患
    Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • 配列類似性
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Anti-XBP1 antibody 画像

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human breast tissue using ab85546 at a dilution of 5µg/ml.
  • Anti-XBP1 antibody (ab85546) at 1 µg/ml + nuclear HeLa lysate in RIPA buffer at 35 µg

    Predicted band size : 40 kDa

Anti-XBP1 antibody (ab85546) 使用論文

This product has been referenced in:
  • Piret SE  et al. A mouse model for inherited renal fibrosis associated with endoplasmic reticulum stress. Dis Model Mech 10:773-786 (2017). IHC-P ; Mouse . Read more (PubMed: 28325753) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-buffer
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 11 2015

Thank you very much for your call yesterday and for forwarding this dog sequence to me.

Looking at the alignment results, I do think it is quite possible for ab37152 to cross-react with your dog samples. I'm sending your information to Cedarl...

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Thank you for your call today and for your interest in our XBP1 antibodies. Please see below for information about your testing code, and let me know if you have any questions or if there is anything else that we can do for you.

DISCOUNT CODE...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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