製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20028872Use at an assay dependent dilution (PMID 20028872).
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910).
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • 関連疾患Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • 配列類似性Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Anti-XBP1 antibody 画像

  • Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
    Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
    Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size : 29 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    The arrow next to the image points to the XBP1 band.
  • Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL
  • ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.

  • ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.

    See Abreview

Anti-XBP1 antibody (ab37152) 使用論文

This product has been referenced in:
  • Jiang D  et al. Acridine Derivatives as Inhibitors of the IRE1a-XBP1 Pathway Are Cytotoxic to Human Multiple Myeloma. Mol Cancer Ther 15:2055-65 (2016). Read more (PubMed: 27307600) »
  • Lake AD  et al. The adaptive endoplasmic reticulum stress response to lipotoxicity in progressive human nonalcoholic fatty liver disease. Toxicol Sci 137:26-35 (2014). Read more (PubMed: 24097666) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

投稿 Mar 16 2016

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

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Verified customer

投稿 Mar 15 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 100 ng/ml LPS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

投稿 Jul 04 2014

Thank you for contacting us.

Antibodies ab37152 and ab109221 are equally specific for XBP-1 and they recognise both isoforms of XBP-1 (http://www.uniprot.org/uniprot/P17861).
ab37152 is actually better characterised than ab109221 with 7 sp...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Aug 28 2012

Thank you for your enquiry.

Here is an isotype control antibody you can use with ab37152:

http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html

I hope this is helpful. Please contact me again if you have any further ques...

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Thank you for contacting us.

The correct antibody for detection of both splice forms (as described at the UniProt page for the mouse protein, http://www.uniprot.org/uniprot/O35426 ) is ab37152:

Click here (or use the following: ht...

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Thank you for confirming these details and for sending images of your results. I will forward these data to the lab, this will enable us to closely monitor the quality of our products. I am sorry the new lot ab37152 did not perform as stated on the dat...

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Thank you for your call today and for your questions. As we discussed, I would be happy to help resolve the issues with the antibodies that you have purchased. Could you please send the following information about the protocol and results using ab79483...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"