製品の概要
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製品名Anti-XBP1 antibody
XBP1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to XBP1
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アプリケーション適用あり: WBmore details
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種交差性交差種: Rat, Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human XBP1.
(Peptide available as ab30769.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, A431 Whole Cell, Rat Testis Tissue
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab28715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
追加情報
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). We believe this band represents the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
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関連疾患Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
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配列類似性Belongs to the bZIP family.
Contains 1 bZIP domain. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all -
別名
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
Anti-XBP1 antibody 画像
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Western blot - XBP1 antibody (ab28715)All lanes : Anti-XBP1 antibody (ab28715) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Testis (Rat) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
プロトコール
Anti-XBP1 antibody (ab28715) 使用論文
ab28715 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"