製品の概要

  • 製品名
    Anti-XBP1 antibody
    XBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to XBP1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human XBP1.

    Immunogen の所有権に関して

    (Peptide available as ab30769.)

  • ポジティブ･コントロール
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, A431 Whole Cell, Rat Testis Tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). We believe this band represents the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能
      Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
    • 関連疾患
      Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
    • 配列類似性
      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
      see all
    • 別名
      • Tax responsive element binding protein 5 antibody
      • Tax-responsive element-binding protein 5 antibody
      • TREB5 antibody
      • X box binding protein 1 antibody
      • X box binding protein 2 antibody
      • X-box-binding protein 1 antibody
      • XBP 1 antibody
      • XBP-1 antibody
      • XBP1 antibody
      • XBP1_HUMAN antibody
      • XBP2 antibody
      see all

    画像

    • Western blot - Anti-XBP1 antibody (ab28715)
      Western blot - Anti-XBP1 antibody (ab28715)
      All lanes : Anti-XBP1 antibody (ab28715) at 1 µg/ml

      Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Testis (Rat) Tissue Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 35 kDa (why is the actual band size different from the predicted?)


      Exposure time: 8 minutes


      We believe the 35 kDa band corresponds to the protein product of the unprocessed XBP1 mRNA that is present in the absence of a stress response.

    プロトコール

    参考文献

    ab28715 has not yet been referenced specifically in any publications.

    ab28715 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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