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Our Abpromise guarantee covers the use of ab17204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 18485100|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with Human XAF1 peptide (ab39906). A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.|
|IHC-P||Use at an assay dependent concentration.|
Western blot analysis of XAF1 in human spleen lysate with ab17204 at
Lane A: 0.5 ug/ml
Lane B: 1ug/ml
Lane C: 2ug/mlHigher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC