XAC Fluorescent ligand (Red) (ab118164)

製品の概要

  • 製品名XAC Fluorescent ligand (Red)
  • 製品の詳細

    Fluorescent A3 antagonist

  • 生理活性の詳細Fluorescent adenosine A3 antagonist (pKD values are 6.57, 6.74 and 8.10 at A1, A2A and A3 respectively). Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics. Antagonizes the activity of NECA (ab120440) in vitro.

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • 精製度> 97%

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab118164 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Small Molecule Fluorescent Optical Detection Use at an assay dependent concentration.
    Receptor Localization Use at an assay dependent concentration.
    Fluorescent Cell Imaging Use at an assay dependent concentration.

    XAC Fluorescent ligand (Red) 画像

    • Left: ab118164 ligand (100 nM) binding to live CHO cells expressing adenosine A3 receptors.
      Right: Binding blocked by the unlabelled competitor XAC (10 µM). Nuclei have been counter-stained with Hoechst.
    • To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
      The apparent KD at A1 receptor was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
      Antagonist -log KD values for A1 is 6.57.
    • To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
      The apparent KD at A2A receptor was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
      the Antagonist -log KD values for A2A is 6.74.
    • To determine the apparent KD for ab118164, cells were treated with varying concentrations of NECA alone, or in the presence of 1 µM ab118164, and the cyclic AMP-induced expression of SPAP measured.
      The apparent KD at A3 receptors was calculated from the rightward shift of the agonist response curve in the presence of ab118164, compared to the response curve for the agonist alone, for each receptor-expressing cell line.
      The Antagonist -log KD value for A3 is 8.1.

    XAC Fluorescent ligand (Red) (ab118164) 使用論文

    ab118164 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.

    I have included a pdf file which contains the pKd values for the products as you have asked. This information and more is also available
    on the individual product pages on the abcam website (e.g. http://www...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"