Anti-Wwp2 抗体 (ab103527)
Key features and details
- Rabbit polyclonal to Wwp2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Wwp2 antibody -
製品の詳細
Rabbit polyclonal to Wwp2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Wwp2 aa 200-300.
Database link: O00308 -
ポジティブ・コントロール
- HeLa, 293T, Jurkat and mouse NIH3T3 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab103527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
Wwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 11060 Human
- Entrez Gene: 66894 Mouse
- Omim: 602308 Human
- SwissProt: O00308 Human
- SwissProt: Q9DBH0 Mouse
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別名
- AIP2 antibody
- Atrophin 1 interacting protein 2 antibody
- NEDD4 like E3 ubiquitin protein ligase antibody
see all
画像
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All lanes : Anti-Wwp2 antibody (ab103527) at 0.1 µg/ml
Lane 1 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 99 kDa
Exposure time: 30 seconds -
All lanes : Anti-Wwp2 antibody (ab103527) at 0.1 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : 293T cell lysate at 5 µg
Lane 4 : HeLa cell lysate at 50 µg
Lane 5 : Jurkat cell lysate at 50 µg
Predicted band size: 99 kDa
Exposure time: 30 secondsCells were prepared using NETN lysis buffer.
Detection: Chemiluminescence
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ab103527 at 1 used at 6 µg/reaction immunoprecipitating Wwp2 in HEK293T cell lysate.
Lane 1: ab103527 + HEK293T cell lysate
Lane 2: Rabbit anti-WWP2 Antibody instead of ab103527 in HEK293T cell lysate
Lane 3: Control IgG instead of ab32562 in HEK293T cell lysate.
For blotting ab103527 was used at 1 µg/mL.
Detection: Chemiluminescence
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab103527 は 15 報の論文で使用されています。
- He X et al. FNDC5/irisin facilitates muscle-adipose-bone connectivity through ubiquitination-dependent activation of runt-related transcriptional factors RUNX1/2. J Biol Chem 298:101679 (2022). PubMed: 35124008
- Qin Y et al. WWP2 overexpression inhibits the antitumor effects of doxorubicin in hepatocellular carcinoma. Cell Biol Int 46:1682-1692 (2022). PubMed: 35880837
- Lu X et al. The role of E3 ubiquitin ligase WWP2 and the regulation of PARP1 by ubiquitinated degradation in acute lymphoblastic leukemia. Cell Death Discov 8:421 (2022). PubMed: 36257929
- Cerny O et al. CD97 stabilises the immunological synapse between dendritic cells and T cells and is targeted for degradation by the Salmonella effector SteD. PLoS Pathog 17:e1009771 (2021). PubMed: 34314469
- Zhang W et al. ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2. FEBS Lett 595:169-182 (2021). PubMed: 33107021