製品の概要

  • 製品名Anti-WWP1 antibody
    WWP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to WWP1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 200 - 250 of Human WWP1 (NP_008944.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7 to 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab104440 is affinity purified using an epitope specific to WWP1 immobilized on solid support
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab104440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.
  • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • AIP 5 antibody
    • AIP5 antibody
    • Atropin-1-interacting protein 5 antibody
    • hSDRP1 antibody
    • Nedd 4 like ubiquitin protein ligase antibody
    • nedd4 like antibody
    • NEDD4-like E3 ubiquitin-protein ligase WWP1 antibody
    • TGIF interacting ubiquitin ligase 1 antibody
    • Tiul1 antibody
    • WW domain containing E3 ubiquitin protein ligase 1 antibody
    • WW domain-containing protein 1 antibody
    • WWP 1 antibody
    see all

Anti-WWP1 antibody 画像

  • All lanes : Anti-WWP1 antibody (ab104440) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 105 kDa


    Exposure time : 30 seconds
  • Detection of Human WWP1 by Western blot of immunoprecipitates prepared from HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab104440 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.

    Detection by chemiluminescence with an exposure time of 10 seconds.

Anti-WWP1 antibody (ab104440) 使用論文

ab104440 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"