Anti-WWOX (phospho Y33) 抗体 (ab78210)

製品の概要

  • 製品名Anti-WWOX (phospho Y33) antibody
    WWOX 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to WWOX (phospho Y33)
  • 特異性This antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human WWOX around the phosphorylation site of tyrosine 33 (W-V-YP-Y-A).

  • ポジティブ・コントロール
    • Extracts from HepG2 cells. Human colon carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.

ターゲット情報

  • 機能Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • 組織特異性Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • 関連疾患Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
    Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
  • 配列類似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 2 WW domains.
  • ドメインThe WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
  • 翻訳後修飾Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
    Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
  • 細胞内局在Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5330426P09Rik antibody
    • 9030416C10Rik antibody
    • Aberrant WW domain-containing oxidoreductase antibody
    • D16S432E antibody
    • EC 1.1.1.- antibody
    • EIEE28 antibody
    • FOR antibody
    • FRA16D antibody
    • Fragile site FRA16D oxidoreductase antibody
    • Fragile site FRA16D Oxireductase antibody
    • HHCMA56 antibody
    • MGC55975 antibody
    • PRO0128 antibody
    • Putative oxidoreductase antibody
    • SCAR12 antibody
    • SDR41C1 antibody
    • Short chain dehydrogenase/reductase family 41C, member 1 antibody
    • WOX1 antibody
    • WW domain containing oxidoreductase antibody
    • WW domain-containing oxidoreductase antibody
    • WW domain-containing protein WWOX antibody
    • wwox antibody
    • WWOX_HUMAN antibody
    • zgc:55975 antibody
    see all

Anti-WWOX (phospho Y33) antibody 画像

  • All lanes : Anti-WWOX (phospho Y33) antibody (ab78210) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins) plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ab78210 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

Anti-WWOX (phospho Y33) antibody (ab78210) 使用論文

ab78210 has not yet been referenced specifically in any publications.

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