製品の概要

  • 製品名Anti-WSTF antibody [EP1704Y]
    WSTF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1704Y] to WSTF
  • アプリケーション適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human WSTF.

  • ポジティブ・コントロール
    • HeLa cell lysate, human lymphoma tissue or HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/15000 - 1/20000. Detects a band of approximately 185 kDa (predicted molecular weight: 171 kDa).
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
    • 組織特異性Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
    • 関連疾患Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 配列類似性Belongs to the WAL family. BAZ1B subfamily.
      Contains 1 bromo domain.
      Contains 1 DDT domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 WAC domain.
    • 発生段階Expressed at equal levels in 19-23 weeks old fetal tissues.
    • ドメインThe N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
      The bromo domain mediates the specific interaction with acetylated histones.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.
    • Information by UniProt
    • 参照データベース
    • 別名
      • baz1b antibody
      • BAZ1B_HUMAN antibody
      • Bromodomain adjacent to zinc finger domain protein 1B antibody
      • hWALP 2 antibody
      • hWALP-2 antibody
      • hWALP2 antibody
      • transcription factor WSTF antibody
      • Tyrosine-protein kinase BAZ1B antibody
      • WALP-2 antibody
      • WALP2 antibody
      • WBRS 9 antibody
      • WBRS-9 antibody
      • WBRS9 antibody
      • WBSC 10 antibody
      • WBSC-10 antibody
      • WBSC10 antibody
      • WBSCR10 antibody
      • WBSCR9 antibody
      • Williams Beuren syndrome chromosome region 9 protein antibody
      • Williams syndrome transcription factor antibody
      • Williams-Beuren syndrome chromosomal region 10 protein antibody
      • Williams-Beuren syndrome chromosomal region 9 protein antibody
      • WSTF antibody
      see all

    Anti-WSTF antibody [EP1704Y] 画像



    • Predicted band size : 171 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: WSTF knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: PC12 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51256 observed at 175 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab51265 was shown to recognize WSTF when WSTF knockout samples were used, along with additional cross-reactive bands. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. ab51256 and ab18058 (loading control to vinculin) were diluted 1/15 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-WSTF antibody [EP1704Y] (ab51256) at 1/20000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size : 171 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • Ab51256 (1:50) staining human WSTF in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab51256 (1:100).
    • Overlay histogram showing HeLa cells stained with ab51256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51256, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Anti-WSTF antibody [EP1704Y] (ab51256) 使用論文

    This product has been referenced in:
    • Vidi PA  et al. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic Acids Res 42:6365-79 (2014). ICC/IF ; Human . Read more (PubMed: 24753406) »
    • Sánchez-Molina S  et al. Role for hACF1 in the G2/M damage checkpoint. Nucleic Acids Res 39:8445-56 (2011). WB . Read more (PubMed: 21745822) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
    Sample Human Cell lysate - nuclear (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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    投稿 Sep 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"