The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Predicted molecular weight: 171 kDa.
Use at an assay dependent dilution.
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Bromodomain adjacent to zinc finger domain protein 1B antibody
hWALP 2 antibody
transcription factor WSTF antibody
Tyrosine-protein kinase BAZ1B antibody
WBRS 9 antibody
WBSC 10 antibody
Williams Beuren syndrome chromosome region 9 protein antibody
Williams syndrome transcription factor antibody
Williams-Beuren syndrome chromosomal region 10 protein antibody
Williams-Beuren syndrome chromosomal region 9 protein antibody
Western blot - Anti-WSTF antibody [BAZ1H4H9] (ab50987)
Predicted band size : 171 kDa Additional bands at : 175 kDa. We are unsure as to the identity of these extra bands.
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: WSTF knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lanes 1 - 3: Merged signal (red and green). Green - ab50987 observed at 175 kDa. Red - loading control, ab176560, observed at 52kDa.
Ab50987 was shown to recognize WSTF in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when WSTF knockout samples were used. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. ab50987 and 176560 (loading control to alpha-tubulin) were diluted 1/100 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - WSTF antibody [BAZ1H4H9] (ab50987)
Anti-WSTF antibody [BAZ1H4H9] (ab50987) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary Anti mouse IgG antibody at 1/2500 dilution
Predicted band size : 171 kDa Observed band size : 171 kDa