製品の概要

  • 製品名Anti-Wnt9a antibody
    Wnt9a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Wnt9a
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Wnt9a.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Lung and Heart tissue lysates, as well as T47D and PANC-1 whole cell lysates. This antibody gave a positive result when used in the following formaldehyde + methanol fixed cell lines: T47D. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 配列類似性Belongs to the Wnt family.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Wnt9a is expressed in gastric cancer cell lines. The protein encoding this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region.
  • 別名
    • MGC138165 antibody
    • MGC141991 antibody
    • Protein Wnt-14 antibody
    • Protein Wnt-9a antibody
    • RP23-378H5.1 antibody
    • Wingless related MMTV integration site 9A antibody
    • Wingless type MMTV integration site 9A antibody
    • Wingless type MMTV integration site family, member 9A antibody
    • wingless-type MMTV integration site family, member 14 antibody
    • Wnt 14 antibody
    • Wnt 9a antibody
    • WNT14 antibody
    • WNT9A antibody
    • WNT9A_HUMAN antibody
    see all

Anti-Wnt9a antibody 画像

  • All lanes : Anti-Wnt9a antibody (ab125957) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : T47D whole cell lysate (ab14899)
    Lane 4 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ab125957 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125957 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) T47D cells at 1ug/ml.

  • IHC image of Wnt9a staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125957, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Wnt9a antibody (ab125957) 使用論文

ab125957 has not yet been referenced specifically in any publications.

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