Anti-Wnt7a 抗体 (ab100792)
Key features and details
- Rabbit polyclonal to Wnt7a
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Wnt7a antibody
Wnt7a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Wnt7a -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Recombinant human Wnt7a protein (ab116171) can be used as positive control in WB. In Western Blot, ab100792 gave a positive signal in Human kidney tissue lysate, human and mouse kidney lysate, HEPg2 cells, COLO 205 whole cell lysate and human colon tumor tissue. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100792の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).
Abcam recommends using 5% BSA as the blocking agent. |
ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa). Abcam recommends using 5% BSA as the blocking agent. |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. -
組織特異性
Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. -
関連疾患
Defects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 749914 Chimpanzee
- Entrez Gene: 101146237 Gorilla
- Entrez Gene: 7476 Human
- Entrez Gene: 22421 Mouse
- Omim: 601570 Human
- SwissProt: Q1KYL1 Chimpanzee
- SwissProt: Q1KYK7 Gorilla
- SwissProt: O00755 Human
see all -
別名
- Protein Wnt-7a antibody
- Protein Wnt-7a precursor antibody
- Proto oncogene Wnt7a protein antibody
see all
画像
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ab100792 staining Wnt7a in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab100792 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Wnt7a antibody (ab100792) at 1 µg/ml
Lane 1 : Human Kidney lysate
Lane 2 : Mouse Kidney lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : COLO 205 whole cell lysate
Lane 5 : Human Colon tumour tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 39 kDaExposure: 4 minutes
Blocking buffer: 2% BSA
Gel type: MOPS
Observed band size: 41 kDa
Additional bands: 50 kDa, 27kDa
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Anti-Wnt7a antibody (ab100792) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab100792 は 19 報の論文で使用されています。
- Yang L et al. Wnt7a promotes the osteogenic differentiation of human mesenchymal stem cells. Int J Mol Med 47:N/A (2021). PubMed: 33846764
- Zhang R et al. lncRNA BCAR4 sponges miR-370-3p to promote bladder cancer progression via Wnt signaling. Int J Mol Med 45:578-588 (2020). PubMed: 31894304
- Taxier LR et al. Dickkopf-1 blocks 17ß-estradiol-enhanced object memory consolidation in ovariectomized female mice. Horm Behav 114:104545 (2019). PubMed: 31228421
- Wang L et al. miR-127 suppresses gastric cancer cell migration and invasion via targeting Wnt7a. Oncol Lett 17:3219-3226 (2019). PubMed: 30867752
- Vallon M et al. A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Cell Rep 25:339-349.e9 (2018). PubMed: 30304675