製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Wnt5b
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within the internal sequence of Human Wnt5b, conjugated to KLH.

  • ポジティブ・コントロール
    • Human prostate tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C.
  • バッファー
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 配列類似性
    Belongs to the Wnt family.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC2648 antibody
    • Protein Wnt-5b antibody
    • Wingless type MMTV integration site family, member 5B antibody
    • WNT 5B antibody
    • WNT 5B protein antibody
    • Wnt family member 5B antibody
    • Wnt5b antibody
    • WNT5B protein antibody
    • WNT5B_HUMAN antibody
    see all

Anti-Wnt5b antibody 画像

  • Human prostate (formalin fixed, paraffin embedded tissue) stained with ab116710 at 2.5 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

プロトコール

Anti-Wnt5b antibody (ab116710) 使用論文

ab116710 has not yet been referenced specifically in any publications.

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Thank you for contacting us.
I am sorry to hear that the antibody is not working as expected.

I have a few more question as well as a suggestion:

1) Your samples are PFA fixed and paraffin embedded?
2) What antibody dilut...

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