製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Wnt4
  • アプリケーション
    適用あり: IHC-P, Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Horse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Wnt4 aa 211-239 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 54361

  • ポジティブ・コントロール
    • WB: Mouse bladder tissue lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: MCF7 cells. Flow Cyt: MB468 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 39 kDa.

ターゲット情報

  • 機能
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
  • 関連疾患
    Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
    Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
    Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330].
  • 配列類似性
    Belongs to the Wnt family.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC123964 antibody
    • Protein Wnt-4 antibody
    • RP23-246F18.1 antibody
    • SERKAL antibody
    • Wingless type MMTV integration site family member 4 precursor antibody
    • WNT 4 antibody
    • WNT 4 protein precursor antibody
    • WNT4 antibody
    • WNT4_HUMAN antibody
    see all

Anti-Wnt4 antibody 画像

  • Immunohistochemical analysis of paraformaldehyde-fixed frozen murine thymus tissue sections, labelling Wnt4 with ab91226 at a dilution of 1/20 incubated for 72 hours at 25°C in a 1% BSA & 3% donkey serum solution in PBST. Permeabilsation was with PBST including 0.1% Triton X-100. Blocking was with the BSA & donkey serum ab91226 was diluted in, incubated for 1 hour at 25°C. The secondary used was a donkey anti-rabbit polyclonal Alexa Fluor® 488

    See Abreview

  • Anti-Wnt4 antibody (ab91226) at 1/1000 dilution + Mouse bladder tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 39 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Wnt4 with ab91226. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.

  • ICC/IF image of ab91226 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow cytometry analysis of MDA-MB468 cells labelling Wnt4 (green) with ab91226 compared to a negative control (blue). A FITC-conjugated goat anit-rabbit IgG was used as the secondary antibody.

Anti-Wnt4 antibody (ab91226) 使用論文

This product has been referenced in:
  • Liu S  et al. Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells. Stem Cells Int 2017:7168687 (2017). WB . Read more (PubMed: 28194187) »
  • Sun X  et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). Read more (PubMed: 28147310) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% Gradient)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Julie Kim

Verified customer

投稿 May 04 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Whole embryo- trigeminal ganglion)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM Citrate Buffer pH6.0
Permeabilization
No
Specification
Whole embryo- trigeminal ganglion
Blocking step
heat-inactivated donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
modified Carnoy's
Username

Dr. Clare Baker

Verified customer

投稿 Nov 23 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (adult thymus)
Permeabilization
Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification
adult thymus
Blocking step
3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Uterus)
Specification
Uterus
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Mukesh Jaiswal

Verified customer

投稿 May 13 2013

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