製品の概要

  • 製品名
    Anti-Wnt3a antibody (Biotin)
    Wnt3a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Wnt3a (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human),>98% pure

  • 特記事項
    The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

製品の特性

  • 製品の状態
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 特記事項(精製)
    A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab54479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Use at a concentration of 0.25 - 1.0 µg/ml. Use 100µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 39 kDa. The detection limit is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
    • 組織特異性
      Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
    • 配列類似性
      Belongs to the Wnt family.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Protein Wnt 3a Precursor antibody
      • Protein Wnt-3a antibody
      • Wingless type MMTV integration site family member 3A antibody
      • Wnt 3a antibody
      • wnt3a antibody
      • WNT3A protein antibody
      • WNT3A_HUMAN antibody
      see all

    プロトコール

    Anti-Wnt3a antibody (Biotin) (ab54479) 使用論文

    This product has been referenced in:
    • Ling L  et al. Synergism between Wnt3a and heparin enhances osteogenesis via a phosphoinositide 3-kinase/Akt/RUNX2 pathway. J Biol Chem 285:26233-44 (2010). Read more (PubMed: 20547765) »
    • Kwon HS  et al. Myocilin is a modulator of Wnt signaling. Mol Cell Biol 29:2139-54 (2009). ELISA ; Mouse . Read more (PubMed: 19188438) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab54479.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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