Biotin Anti-Wnt3a 抗体 (ab54479)
Key features and details
- Biotin Rabbit polyclonal to Wnt3a
- Suitable for: Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Wnt3a antibody
Wnt3a 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Wnt3a -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length protein (Human),>98% pure
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading...
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特記事項(精製)
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54479の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Use 100 µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a. |
特記事項 |
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Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. Use 100 µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
組織特異性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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別名
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab54479 は 2 報の論文で使用されています。
- Ling L et al. Synergism between Wnt3a and heparin enhances osteogenesis via a phosphoinositide 3-kinase/Akt/RUNX2 pathway. J Biol Chem 285:26233-44 (2010). PubMed: 20547765
- Kwon HS et al. Myocilin is a modulator of Wnt signaling. Mol Cell Biol 29:2139-54 (2009). ELISA ; Mouse . PubMed: 19188438