製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Wnt3a
  • アプリケーション
    適用あり: WB, IHC-Fr, IP, IHC - Wholemount, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Chicken, Human, Zebrafish
    交差が予測される動物種: Xenopus laevis, a wide range of other species
  • 免疫原

    Synthetic peptide derived from within residues 250 - 350 of Human Wnt3a.

    (Peptide available as ab23327.)

  • ポジティブ・コントロール
    • In WB, this antibody gave a positive signal when tested against recombinant Wnt3a protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Wnt3a peptide (ab23326) or Human Wnt3a peptide (ab23327).
IHC-Fr Use at an assay dependent concentration. PubMed: 21048974
IP Use at an assay dependent concentration. PubMed: 21740904
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    画像

    • Anti-Wnt3a antibody (ab28472) at 1 µg/ml + Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 39 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.

    • ab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
    • ab28472 staining Wnt3a in the dentate gyrus of rat bain tissue by Immunohistochemistry (Frozen sections).
      Brains were sectioned in the coronal plane at 20 µm. The slides were left in a fume hood to dry overnight, then stored at -70°C. For each rat twelve sections through the dorsal hippocampus were analyzed. For single staining with Wnt3a, sections were incubated with ab28472 at 5 ug/ml in 0.5% Triton with 1% goat serum) overnight in a humidified chamber at room temperature. The following day, after two washes with KPBS, they were incubated in Alexa Fluor 568 goat anti-rabbit (1/200 in 0.5% Triton) for one hour, and washed twice with KPBS and covered slipped.
    • IHC-Wholemount image of Wnt3a (ab28472) on zebrafish larvae. Whole zebrafish larvae were fixed in paraformaldehyde and then permeabilized in proteinase K, before blocking in 5% serum for 1 hour at RT. The image above is a ventral view of the head, showing the ceratohyal cartilage (of the jaw).

      See Abreview

    参考文献

    This product has been referenced in:
    • Zhang Y  et al. MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res 9:136-145 (2017). WB ; Human . Read more (PubMed: 28123640) »
    • Sun X  et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). Read more (PubMed: 28147310) »

    See all 16 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E8.5 mouse embryo sections)
    Specification
    E8.5 mouse embryo sections
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
    Blocking step
    NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 16 2012

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Wnt3a antibody.

    The details you have kindly provided will enable us to investigate...

    Read More
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (whole embryo - 24hpf)
    Specification
    whole embryo - 24hpf
    Username

    Ms. Emerald Butko

    Verified customer

    投稿 Mar 09 2012

    Thank you for contacting Abcam.

    The discount code has been added to your Abreview.

    If there is anything else I can help you with, please let me know.

    Application
    Western blot
    Sample
    Guinea pig Cell lysate - whole cell (brain)
    Loading amount
    30 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 29 2012

    Thank you for your enquiry and interest.

    I regret to inform you that we are unable to change the formulation of this antibody.

    Apologies! If you need any further assistance in the future, please do not hesitate to contact me.

    Thank you for contacting us and I apologize that you did not receive these codes earlier. These codes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in guinea pig...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Whole mount (whole zebrafish larvae))
    Specification
    Whole mount (whole zebrafish larvae)
    Username

    Dr. Chrissy Hammond

    Verified customer

    投稿 Oct 24 2011

    Thank you for your enquiry. I would like to align the immunogen peptide with the canine Wnt3a sequence but I have been unable to locate the canine Wnt3a sequence. If you have the canine Wnt3a sequence, here is the immunogen sequence used to prod...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (liver (human mouse and rat))
    Specification
    liver (human mouse and rat)
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization
    No
    Blocking step
    sequential peroxidase and protein block (prediluated) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Apr 28 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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