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Our Abpromise guarantee covers the use of ab50341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
ab50341 (2µg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
ab50341 at 3.8µg/ml staining Wnt3 in Human breast tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. An AP staining procedure was used.
ab50341 has not yet been referenced specifically in any publications.