製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Wnt2b
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: AVQVMATQDG ANFTAARQGY RRATRTDLVY FDNSPDYCVL DKAAGSLGTA, corresponding to internal sequence amino acids 253-302 of Human Wnt2b.

  • ポジティブ・コントロール
    • Jurkat cell lysate and human lung tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
  • 組織特異性
    Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
  • 配列類似性
    Belongs to the Wnt family.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein Wnt-13 antibody
    • Protein Wnt-2b antibody
    • Wingless type MMTV integration site family, member 13 antibody
    • Wingless type MMTV integration site family, member 2B antibody
    • WNT13 antibody
    • Wnt2b antibody
    • WNT2B_HUMAN antibody
    • XWNT2 antibody
    • XWNT2, Xenopus, homolog of antibody
    see all

画像

  • Anti-Wnt2b antibody (ab50575) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab50575 at 4ug/ml dilution staining human Wnt2b in alveolar cells of human lung by Immunohistochemistry, Paraffin embedded tissue.ab50575 at 4ug/ml dilution staining human Wnt2b in alveolar cells of human lung by Immunohistochemistry, Paraffin embedded tissue.
  • All lanes : Anti-Wnt2b antibody (ab50575) at 1.25 µg/ml

    Lane 1 : Jusrkat cell lysate
    Lane 2 : Jusrkat cell lysate with blocking peptide at 4 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 41 kDa
  • All lanes : Anti-Wnt2b antibody (ab50575) at 1/1000 dilution (in TBST + 5% Milk Powder for 3 hours at 21°C)

    Lane 1 : Whole tissue lysate of Mouse (FVB) liver
    Lane 2 : Whole tissue lysate of Mouse (FVB) liver
    Lane 3 : Whole tissue lysate of Mouse (FVB) liver
    Lane 4 : Whole tissue lysate of Mouse (BL6) liver
    Lane 5 : Lysate of HepG2 clls

    Lysates/proteins at 20 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabiit IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 41 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step : 5% Milk for 16 hours at 4°C

    See Abreview

参考文献

This product has been referenced in:
  • Gong SJ  et al. Upregulation of PP2Ac predicts poor prognosis and contributes to aggressiveness in hepatocellular carcinoma. Cancer Biol Ther 17:151-62 (2016). Read more (PubMed: 26618405) »
  • Richards MH  et al. Dynamic interaction between astrocytes and infiltrating PBMCs in context of neuroAIDS. Glia 63:441-51 (2015). Read more (PubMed: 25331637) »

See all 5 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Sep 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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