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RabMAb

Anti-Wnt16 抗体 [EPR6064] (ab109437)

製品の概要

  • 製品名
    Anti-Wnt16 antibody [EPR6064]
    Wnt16 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6064] to Wnt16
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Wnt16

  • ポジティブ・コントロール
    • LnCaP, HepG2, Raji, and 293T cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値)
    KD = 6.05 x 10 -11 M
    Learn more about KD
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR6064
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能
      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • 組織特異性
      Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
    • 配列類似性
      Belongs to the Wnt family.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Protein Wnt 16 precursor antibody
      • Protein Wnt-16 antibody
      • Wingless type MMTV integration site family member 16 antibody
      • WNT16 antibody
      • WNT16_HUMAN antibody
      see all

    画像

    • All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »
    • Andres-Mateos E  et al. Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels. PLoS One 7:e48884 (2012). WB ; Other . Read more (PubMed: 23155423) »

    See all 2 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Wholemount 3-5 days post fertilisation)
    Specification
    Wholemount 3-5 days post fertilisation
    Username

    Dr. Chrissy Hammond

    Verified customer

    投稿 Jul 23 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver, Spleen)
    Specification
    Liver, Spleen
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 31 2012

    Thank you for this data.

    After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came ...

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    Thank you for contacting us.

    I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased withi...

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    Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence...

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    DISCOUNT CODE: 00000

    Expiration date:04. January 2013

    Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to you...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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