製品の概要

  • 製品名Anti-Wnt11 antibody
    Wnt11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Wnt11
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Wnt11.

    (Peptide available as ab28716.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MDA-MB-361 whole cell lysate. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab31962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Wnt11 peptide (ab28716).

ターゲット情報

  • 機能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 組織特異性Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas.
  • 配列類似性Belongs to the Wnt family.
  • 翻訳後修飾Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HWNT11 antibody
    • Protein Wnt-11 antibody
    • Wingless-related MMTV integration site 11 antibody
    • Wingless-type MMTV integration site family, member 1 antibody
    • Wingless-type MMTV integration site family, member 11 antibody
    • Wingless-type MMTV integration site family, member 11B antibody
    • wnt-11 antibody
    • wnt11 antibody
    • WNT11_HUMAN antibody
    • wnt11b antibody
    see all

Anti-Wnt11 antibody 画像

  • Anti-Wnt11 antibody (ab31962) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 15 µg

    Secondary
    Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab31962 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31962 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Wnt11 antibody (ab31962) 使用論文

This product has been referenced in:
  • Nagy II  et al. Impairment of Wnt11 function leads to kidney tubular abnormalities and secondary glomerular cystogenesis. BMC Dev Biol 16:30 (2016). IHC (PFA fixed) ; Mouse . Read more (PubMed: 27582005) »
  • Zhang P  et al. Activation of Wnt11 by TGF-ß drives mesenchymal gene expression through non-canonical Wnt signaling in renal Epithelial cells. J Biol Chem : (2012). WB . Read more (PubMed: 22556418) »

See all 7 Publications for this product

Product Wall

Abreviews
Application ELISA
Sample Human Cell (Colon cancer)
Specification Colon cancer
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Type Indirect
Username

Ms. Sonakshi Mishra

Verified customer

投稿 Sep 16 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult ovary and uterus)
Permeabilization Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification Adult ovary and uterus
Blocking step 3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 21 2015

Application Western blot
Sample Human Cell lysate - whole cell (MCF7, K562 and A459 cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 40 µg
Specification MCF7, K562 and A459 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 23 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hep G2)
Specification Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 29 2014

Thank you for your message and for kindlyproviding this further information.

The suggestions provided were given as these are our general recommendations for western blot. For further tips, I required further information regarding the sample...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB a...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab111874.

To check the status of the order please contact o...

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Thanks for following up with me.

I'm sorry to hear the replacement antibody did not improve your data. I would be happy to send you a different primary antibody as a replacement. Please just let me know which catalog number you would like and...

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As requested, I have issued a free of charge replacement for one vial of ab96730. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"