Anti-Wnt11 抗体 (ab31962)
Key features and details
- Rabbit polyclonal to Wnt11
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Wnt11 antibody
Wnt11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Wnt11 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow -
免疫原
Synthetic peptide corresponding to Human Wnt11 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab28716) -
ポジティブ・コントロール
- This antibody gave a positive signal in MDA-MB-361 whole cell lysate. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31962の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
組織特異性
Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas. -
配列類似性
Belongs to the Wnt family. -
翻訳後修飾
Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7481 Human
- Entrez Gene: 22411 Mouse
- Entrez Gene: 140584 Rat
- Omim: 603699 Human
- SwissProt: O96014 Human
- SwissProt: P48615 Mouse
- Unigene: 108219 Human
- Unigene: 22182 Mouse
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別名
- HWNT11 antibody
- Protein Wnt-11 antibody
- Wingless-related MMTV integration site 11 antibody
see all
画像
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ab31962 staining Wnt11 in HaCat cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31962 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-Wnt11 antibody (ab31962) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 15 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab31962 は 18 報の論文で使用されています。
- Liu Y et al. Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma. Cell Rep 30:771-782.e6 (2020). PubMed: 31968252
- Li K et al. Tanshinone IIA promotes cardiac differentiation and improves cell motility by modulating the Wnt/ß-catenin signaling pathway. Mol Med Rep 22:1839-1846 (2020). PubMed: 32582982
- Kawarazaki W et al. Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency. J Clin Invest 130:4152-4166 (2020). PubMed: 32597829
- Choi EY et al. Wnt5a and Wnt11 as acute respiratory distress syndrome biomarkers for severe acute respiratory syndrome coronavirus 2 patients. Eur Respir J 56:N/A (2020). PubMed: 32859680
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700