製品の概要

  • 製品名Anti-Wnt10b antibody
    Wnt10b 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Wnt10b
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Wnt10b.

    (Peptide available as ab66720.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human MCF7 Whole Cell Lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human Wnt10b peptide (ab66720).

ターゲット情報

  • 機能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 組織特異性Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
  • 関連疾患Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
  • 配列類似性Belongs to the Wnt family.
  • 発生段階Infant brain has higher levels of WNT10B than adult brain.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein Wnt-10b antibody
    • Protein Wnt-12 antibody
    • SHFM6 antibody
    • wingless type MMTV integration site family, member 10B antibody
    • WN10B_HUMAN antibody
    • WNT 10B protein antibody
    • WNT 12 antibody
    • Wnt10b antibody
    • wnt12 antibody
    see all

Anti-Wnt10b antibody 画像

  • Anti-Wnt10b antibody (ab66721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ICC/IF image of ab66721 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66721, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, and MCF-7 cells at 1µg/ml, and in 4% pfa fixed (10 min) HeLa, HepG2, MCF-7 cells at 1µg/ml.

Anti-Wnt10b antibody (ab66721) 使用論文

ab66721 has not yet been referenced specifically in any publications.

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