製品の概要

  • 製品名
    Anti-Wnt1 antibody
    Wnt1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Wnt1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • ポジティブ･コントロール
    • Breast carcinoma. NIH/3T3 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

ターゲット情報

  • 機能
    Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • 配列類似性
    Belongs to the Wnt family.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
    • 別名
      • BMND16 antibody
      • INT1 antibody
      • OI15 antibody
      • oncogene Int1 antibody
      • Proto oncogene protein Wnt 1 antibody
      • Proto-oncogene Int-1 homolog antibody
      • Proto-oncogene Wnt-1 antibody
      • Wingless type MMTV integration site family member 1 antibody
      • wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody
      • Wnt 1 antibody
      • wnt1 antibody
      • WNT1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Wnt1 antibody (ab15251)
      Western blot - Anti-Wnt1 antibody (ab15251)
      Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)

      ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

    • Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (ab15251)
      Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (ab15251)

      ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)
      Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);

    参考文献

    This product has been referenced in:

    See all 33 Publications for this product

    ab15251 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    13 reviews or Q&A

    10
    Votes: Highest First

    ELISA abreview for Anti-Wnt1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Recombinant protein (CDK8/CycC)
    Specification
    CDK8/CycC
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
    Type
    Direct
    Read More
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    Abcam user community

    Verified customer

    投稿 Mar 14 2013

    IHC on PFA-fixedfrozen tissue sections is not working, though IHC on paraffin-embedded tissue is.

    Thank you for contacting us.

    It may be, as we discussed, that the PFA fixation of the the frozen tissue is the reason the immunostain is not working. I am attaching three papers that deal with antigen retrieval for IHC of frozen tissue. Two ...

    Read More

    Western blot abreview for Anti-Wnt1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    35 µg
    Sample
    Mouse Cell lysate - whole cell (primary mouse pancreatic cancer cell lines)
    Specification
    primary mouse pancreatic cancer cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    投稿 Dec 15 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Wnt1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (PanIN lesions Pancreas)
    Specification
    PanIN lesions Pancreas
    Fixative
    Acetone
    Permeabilization
    Yes - PBS + Triton 0.025%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Dec 06 2010

    Western blot abreview for Anti-Wnt1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (CaPan1 and Panc1)
    Loading amount
    35 µg
    Specification
    CaPan1 and Panc1
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    投稿 Dec 04 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Wnt1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreatic tumor)
    Specification
    pancreatic tumor
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    Yes - PBST
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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    投稿 Oct 04 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Wnt1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic tumor)
    Specification
    Pancreatic tumor
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    Yes - PBST
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Oct 04 2010

    Western blot abreview for Anti-Wnt1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Podocytes)
    Loading amount
    30 µg
    Specification
    Podocytes
    Treatment
    5ng/ml TGF-beta 5min to 4hr
    Gel Running Conditions
    Reduced Denaturing (85 gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Feb 24 2009

    I am interested in your ab15251 Wnt1 antibody which reacts with human, mouse, and rat proteins. I am curious as to whether this could interact with the zebrafish wnt1 protein. Unfortunately, the amino acid sequence is considered to be commercially sens...

    Read More

    Thank you for your enquiry. The immunogen sequence used to raise this product is highly similar to the zebra fish, exhibiting a difference of 2 out of 17aa. If you have any further questions then please get back in touch with me.

    Thank you for helping out.To be accurate, Where the CELLS (not lysates) transfected with Wnt-1 prior to lysis? I am sure you will say no, but I had to clarify.

    Thanks again for your email. No, the cells were not transfected with Wnt1. The lysate was used to detect the endogenous protein. If you have any additional questions, just let us know!

    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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