製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Wnt1
  • アプリケーション
    適用あり: ELISA, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • ポジティブ・コントロール
    • Breast carcinoma. NIH/3T3 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

ターゲット情報

  • 機能
    Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • 配列類似性
    Belongs to the Wnt family.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BMND16 antibody
    • INT1 antibody
    • OI15 antibody
    • oncogene Int1 antibody
    • Proto oncogene protein Wnt 1 antibody
    • Proto-oncogene Int-1 homolog antibody
    • Proto-oncogene Wnt-1 antibody
    • Wingless type MMTV integration site family member 1 antibody
    • wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody
    • Wnt 1 antibody
    • wnt1 antibody
    • WNT1_HUMAN antibody
    see all

画像

  • Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

  • ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);

参考文献

This product has been referenced in:
  • Kong LY  et al. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/ß-catenin signaling pathway. Oncotarget 8:15507-15519 (2017). WB ; Human . Read more (PubMed: 28099945) »
  • Chen Y  et al. miRNA-148a serves as a prognostic factor and suppresses migration and invasion through Wnt1 in non-small cell lung cancer. PLoS One 12:e0171751 (2017). Read more (PubMed: 28199399) »

See all 29 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (CDK8/CycC)
Specification
CDK8/CycC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Type
Direct
Username

Abcam user community

Verified customer

投稿 Mar 14 2013

Thank you for contacting us.

It may be, as we discussed, that the PFA fixation of the the frozen tissue is the reason the immunostain is not working. I am attaching three papers that deal with antigen retrieval for IHC of frozen tissue. Two ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
35 µg
Sample
Mouse Cell lysate - whole cell (primary mouse pancreatic cancer cell lines)
Specification
primary mouse pancreatic cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (PanIN lesions Pancreas)
Specification
PanIN lesions Pancreas
Fixative
Acetone
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

投稿 Dec 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (CaPan1 and Panc1)
Loading amount
35 µg
Specification
CaPan1 and Panc1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreatic tumor)
Specification
Pancreatic tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Podocytes)
Loading amount
30 µg
Specification
Podocytes
Treatment
5ng/ml TGF-beta 5min to 4hr
Gel Running Conditions
Reduced Denaturing (85 gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 24 2009

Thank you for your enquiry. The immunogen sequence used to raise this product is highly similar to the zebra fish, exhibiting a difference of 2 out of 17aa. If you have any further questions then please get back in touch with me.

Thanks again for your email. No, the cells were not transfected with Wnt1. The lysate was used to detect the endogenous protein. If you have any additional questions, just let us know!

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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