製品の概要
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製品名Anti-Wnt1 antibody
Wnt1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Wnt1
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由来種Rabbit
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アプリケーション適用あり: ELISA, IHC-P, IHC-Fr, WBmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).
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ポジティブ･コントロール
- Breast carcinoma. NIH/3T3 cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab15251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
ターゲット情報
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機能Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
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配列類似性Belongs to the Wnt family.
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細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 7471 Human
- Entrez Gene: 22408 Mouse
- Entrez Gene: 24881 Rat
- Omim: 164820 Human
- SwissProt: P04628 Human
- SwissProt: P04426 Mouse
- Unigene: 248164 Human
- Unigene: 1123 Mouse
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別名
- BMND16 antibody
- INT1 antibody
- OI15 antibody
see all
画像
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Western blot - Anti-Wnt1 antibody (ab15251)Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate
Predicted band size: 41 kDa
Observed band size: 41 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (ab15251)ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
参考文献
This product has been referenced in:
- Kong LY et al. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/ß-catenin signaling pathway. Oncotarget 8:15507-15519 (2017). WB ; Human . Read more (PubMed: 28099945) »
- Chen Y et al. miRNA-148a serves as a prognostic factor and suppresses migration and invasion through Wnt1 in non-small cell lung cancer. PLoS One 12:e0171751 (2017). Read more (PubMed: 28199399) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"