Anti-Wnt1 抗体 (ab15251)
Key features and details
- Rabbit polyclonal to Wnt1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Wnt1 antibody
Wnt1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Wnt1 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human Wnt1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P04628 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15251の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P | (2) |
1/100.
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WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
1/100. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7471 Human
- Entrez Gene: 22408 Mouse
- Entrez Gene: 24881 Rat
- Omim: 164820 Human
- SwissProt: P04628 Human
- SwissProt: P04426 Mouse
- Unigene: 248164 Human
- Unigene: 1123 Mouse
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別名
- BMND16 antibody
- INT1 antibody
- OI15 antibody
see all
画像
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Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate
Predicted band size: 41 kDa
Observed band size: 41 kDa -
ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
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ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (142)
ab15251 は 142 報の論文で使用されています。
- Yu Y et al. High Fat Diet Induces Kidney Injury via Stimulating Wnt/β-Catenin Signaling. Front Med (Lausanne) 9:851618 (2022). PubMed: 35462998
- Zhang Y et al. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/β-catenin signaling in obstructed kidneys in vivo. J Biol Chem 298:102010 (2022). PubMed: 35525270
- Huang X et al. Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis. BMC Endocr Disord 22:189 (2022). PubMed: 35869471
- Mounir MMF et al. Amelogenin as a regenerative endodontic molecule for immature teeth with apical periodontitis. An experimental study. J Oral Biol Craniofac Res 12:721-726 (2022). PubMed: 36097610
- Xu J et al. Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling. Am J Cancer Res 12:5552-5563 (2022). PubMed: 36628278