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Our Abpromise guarantee covers the use of ab109822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. ab109822 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Wnt1.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Wnt1 (Biotin) (ab84243). ab109822 in conjunction with ab84243 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Wnt1.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa. The detection limit for recombinant Human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
ab109822 has not yet been referenced specifically in any publications.