製品の概要

  • 製品名Anti-WNK1 antibody
    WNK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to WNK1
  • アプリケーション適用あり: WB, ELISA, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from huma WNK1 around the phosphorylation site of threonine 58 (R-H-T-M-D).

  • ポジティブ・コントロール
    • EGF-treated 293 cell extract; human breast carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Predicted molecular weight: 251 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20398666

ターゲット情報

  • 機能Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • 組織特異性Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • 関連疾患Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Erythrocyte 65 kDa protein antibody
    • HSAN2 antibody
    • HSN2 antibody
    • hWNK1 antibody
    • KDP antibody
    • KIAA0344 antibody
    • Kinase deficient protein antibody
    • MGC163339 antibody
    • MGC163341 antibody
    • p65 antibody
    • PRKWNK1 antibody
    • Prostate derived sterile 20 like kinase antibody
    • Protein kinase lysine deficient 1 antibody
    • Protein kinase lysine-deficient 1 antibody
    • Protein kinase with no lysine 1 antibody
    • PSK antibody
    • Serine/threonine protein kinase WNK1 antibody
    • Serine/threonine-protein kinase WNK1 antibody
    • With no K antibody
    • WNK lysine deficient protein kinase 1 antibody
    • WNK1 antibody
    • WNK1_HUMAN antibody
    see all

Anti-WNK1 antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with ab53151 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-WNK1 antibody (ab53151) at 1/300 dilution

    Lane 1 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with no peptide
    Lane 2 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with peptide


    Predicted band size : 251 kDa

Anti-WNK1 antibody (ab53151) 使用論文

This product has been referenced in:
  • Park HW  et al. Dynamic regulation of CFTR bicarbonate permeability by [Cl-]i and its role in pancreatic bicarbonate secretion. Gastroenterology 139:620-31 (2010). IHC-Fr ; Human . Read more (PubMed: 20398666) »

See 1 Publication for this product

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I'm really sorry but we unfortunately don't. Sorry I cannot help you more.

Thank you for your enquiry. I would predict that a cytoplasmic lysate of human breast tumor tissue will have some WNK1 protein as in tissue sections of human breast carcinoma this protein was detected with the antibody. However, as the lysate is a ho...

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