製品の概要

  • 製品名Anti-WISP3 antibody
    WISP3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to WISP3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human WISP3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human kidney tissue lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab103263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.
  • 組織特異性Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes.
  • 関連疾患Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]. PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging.
  • 配列類似性Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCN 6 antibody
    • CCN family member 6 antibody
    • CCN6 antibody
    • CYR61 antibody
    • LIBC antibody
    • Lost in inflammatory breast cancer tumor suppressor protein antibody
    • MGC125987 antibody
    • MGC125988 antibody
    • MGC125989 antibody
    • OTTHUMP00000040421 antibody
    • PPAC antibody
    • PPD antibody
    • UNQ462/PRO790/PRO956 antibody
    • WISP 3 antibody
    • WISP-3 antibody
    • WISP3 antibody
    • WISP3_HUMAN antibody
    • WNT 1 inducible signaling pathway protein 3 antibody
    • Wnt 1 signaling pathway protein 3 antibody
    • WNT1 inducible signaling pathway protein 3 antibody
    • WNT1 inducible signaling pathway protein 3 precursor antibody
    • WNT1-inducible-signaling pathway protein 3 antibody
    see all

Anti-WISP3 antibody 画像

  • Anti-WISP3 antibody (ab103263) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab103263 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103263 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-WISP3 antibody (ab103263) 使用論文

ab103263 has not yet been referenced specifically in any publications.

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