Anti-WISP3 抗体 (ab103263)
Key features and details
- Rabbit polyclonal to WISP3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WISP3 antibody
WISP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WISP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human WISP3 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab115244) -
ポジティブ・コントロール
- Recombinant human WISP3 protein (ab50049) can be used as a positive control in WB. This antibody gave a positive signal in Human kidney tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab103263の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 37 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Appears to be required for normal postnatal skeletal growth and cartilage homeostasis. -
組織特異性
Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes. -
関連疾患
Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]. PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging. -
配列類似性
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 8838 Human
- Entrez Gene: 327743 Mouse
- Omim: 603400 Human
- SwissProt: O95389 Human
- SwissProt: D3Z5L9 Mouse
- Unigene: 558428 Human
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別名
- CCN 6 antibody
- CCN family member 6 antibody
- CCN6 antibody
see all
画像
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Anti-WISP3 antibody (ab103263) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
WISP3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
ICC/IF image of ab103263 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103263 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab103263 は 1 報の論文で使用されています。
- Hara C et al. Involvement of multiple CCN family members in platelets that support regeneration of joint tissues. Mod Rheumatol 26:940-949 (2016). PubMed: 26915735