The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
May have direct activity on the actin cytoskeleton. Induces actin polymerization and redistribution. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
Belongs to the verprolin family. Contains 1 WH2 domain.
Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
Cytoplasmic vesicle. Cytoplasm > cytoskeleton. Vesicle surfaces and along actin tails. Co-localized with actin stress fibers. When co-expressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL.
WAS/WASL interacting protein family member 1 antibody
WAS/WASL-interacting protein family member 1 antibody
WASP-interacting protein antibody
Wiskott-Aldrich syndrome protein interacting protein antibody
Wiskott-Aldrich syndrome protein-interacting protein antibody
Western blot - WIPF1 antibody (ab98176)
All lanes : Anti-WIPF1 antibody (ab98176) at 1 µg/ml
Lane 1 : Tonsil (Human) Tissue Lysate Lane 2 : Platelet (Human) Whole Cell Lysate Lane 3 : Human spleen tissue lysate - total protein (ab29699) Lane 4 : Human placenta tissue lysate - total protein (ab29745) Lane 5 : Human colon tissue lysate - total protein (ab30051) Lane 6 : Human thymus tissue lysate - total protein (ab30146) Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 51 kDa Observed band size : 51 kDa