製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to WIPF1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human WIPF1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Jurkat whole cell lysate as well as the following Human tissue lysates: Tonsil; Platelet; Spleen; Placenta; Colon; Thymus.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 機能
    May have direct activity on the actin cytoskeleton. Induces actin polymerization and redistribution. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
  • 組織特異性
    Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
  • 配列類似性
    Belongs to the verprolin family.
    Contains 1 WH2 domain.
  • ドメイン
    Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
  • 細胞内局在
    Cytoplasmic vesicle. Cytoplasm > cytoskeleton. Vesicle surfaces and along actin tails. Co-localized with actin stress fibers. When co-expressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI115543 antibody
    • D2Ertd120e antibody
    • MGC111041 antibody
    • Protein PRPL-2 antibody
    • PRPL 2 antibody
    • PRPL-2 protein antibody
    • WAS/WASL interacting protein family member 1 antibody
    • WAS/WASL-interacting protein family member 1 antibody
    • WAS2 antibody
    • WASP-interacting protein antibody
    • WASPIP antibody
    • WIP antibody
    • Wipf1 antibody
    • WIPF1_HUMAN antibody
    • Wiskott-Aldrich syndrome protein interacting protein antibody
    • Wiskott-Aldrich syndrome protein-interacting protein antibody
    see all

画像

  • All lanes : Anti-WIPF1 antibody (ab98176) at 1 µg/ml

    Lane 1 : Tonsil (Human) Tissue Lysate
    Lane 2 : Platelet (Human) Whole Cell Lysate
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Human placenta tissue lysate - total protein (ab29745)
    Lane 5 : Human colon tissue lysate - total protein (ab30051)
    Lane 6 : Human thymus tissue lysate - total protein (ab30146)
    Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa


    Exposure time : 2 minutes

プロトコール

参考文献

ab98176 has not yet been referenced specifically in any publications.

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