WI-38 nuclear extract lysate (forskolin stimulated) (ab14912)

製品の概要

• 製品名WI-38 nuclear extract lysate (forskolin stimulated)
  WI 38 ライゼート 製品一覧
• 製品の詳細
  WI-38 nuclear extract lysate
• 特記事項The WI-38 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay.

製品の特性

• 製品の状態Liquid
• 保存方法Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
• バッファーpH: 7.50
  Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
  Note: With Protease inhibitor
• Concentration information loading...
• 研究分野
  • Kits/ Lysates/ Other
  • Lysates
  • Nuclear Lysates
  • Human
  • Other

WI-38 nuclear extract lysate (forskolin stimulated) 画像

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  WI-38 Nuclear Extract Forskolin Stimulated

  Each lot has been tested for CREB activation by using TransAM™ pCREB Kits. The signal intensity for CREB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 3. This ratio may vary depending on the basal level of CREB activation in a given cell type.