製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to WHSC2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human WHSC2 protein (NP_005654.2).

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate; WHSC2 transfected 293T cell lysate ICC/IF: HeLa cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
  • 組織特異性
    Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
  • 配列類似性
    Belongs to the NELF-A family.
    Contains 1 HDAg-like domain.
  • ドメイン
    The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ10442 antibody
    • FLJ25112 antibody
    • Negative elongation factor A antibody
    • Negative elongation factor polypeptide A antibody
    • NELF A antibody
    • NELF-A antibody
    • NELFA antibody
    • NELFA_HUMAN antibody
    • P/OKcl.15 antibody
    • P/OKcl15 antibody
    • WHSC 2 antibody
    • Whsc2 antibody
    • Wolf Hirschhorn syndrome candidate 2 antibody
    • Wolf Hirschhorn syndrome candidate 2 protein antibody
    • Wolf-Hirschhorn syndrome candidate 2 protein antibody
    see all

Anti-WHSC2 antibody 画像

  • Anti-WHSC2 antibody (ab88870) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • All lanes : Anti-WHSC2 antibody (ab88870) at 1 µg/ml

    Lane 1 : WHSC2 transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab88870, at 10 µg/ml, staining WHSC2 in HeLa cell by Immunofluorescence.

Anti-WHSC2 antibody (ab88870) 使用論文

ab88870 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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