Anti-WRNIP1/WHIP 抗体 (ab99316)
Key features and details
- Rabbit polyclonal to WRNIP1/WHIP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WRNIP1/WHIP antibody
WRNIP1/WHIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WRNIP1/WHIP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human WRNIP1/WHIP aa 400-500. NP_064520.2
Database link: Q96S55 -
ポジティブ・コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab99316 was affinity purified using an epitope specific to WRNIP1/WHIP immobilized on solid support -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99316の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger. -
翻訳後修飾
Sumoylated by SUMO1 and SUMO2/3. -
細胞内局在
Nucleus. Colocalizes with WRN in granular structures in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56897 Human
- Entrez Gene: 282835 Rat
- Omim: 608196 Human
- SwissProt: Q96S55 Human
- SwissProt: Q8CG07 Rat
- Unigene: 236828 Human
- Unigene: 8783 Rat
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別名
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
see all
画像
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Anti-WRNIP1/WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg
Secondary
IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WRNIP1/WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-WRNIP1/WHIP antibody (ab99316) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds -
Detection of WRNIP1/WHIP by Western Blot of Immunprecipitate.
ab99316, at 1 µg/ml, staining WRNIP1/WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab99316 は 1 報の論文で使用されています。
- Höllmüller E et al. Site-specific ubiquitylation acts as a regulator of linker histone H1. Nat Commun 12:3497 (2021). PubMed: 34108453