1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
Belongs to the AAA ATPase family. Contains 1 UBZ-type zinc finger.
Sumoylated by SUMO1 and SUMO2/3.
Nucleus. Colocalizes with WRN in granular structures in the nucleus.
All lanes : Anti-WHIP antibody (ab99316) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
Immunoprecipitation - WHIP antibody (ab99316)
Detection of WHIP by Western Blot of Immunprecipitate.
ab99316, at 1 µg/ml, staining WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.