製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to WHIP
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    SRESYDAPPTPSGAC

    conjugated to KLH, corresponding to amino acids 107-120 of Human WHIP.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報
    ELISA: 1/5000 - 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 17888034).
    WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
    • 組織特異性
      Ubiquitously expressed.
    • 配列類似性
      Belongs to the AAA ATPase family.
      Contains 1 UBZ-type zinc finger.
    • 翻訳後修飾
      Sumoylated by SUMO1 and SUMO2/3.
    • 細胞内局在
      Nucleus. Colocalizes with WRN in granular structures in the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATPase WRNIP 1 antibody
      • ATPase WRNIP1 antibody
      • bA420G6.2 antibody
      • FLJ22526 antibody
      • Putative helicase RUVBL antibody
      • RP11 420G6.2 antibody
      • Werner helicasae interacting protein 1 antibody
      • Werner helicasae interacting protein antibody
      • Werner helicase interacting protein isoform 2 antibody
      • Werner helicase-interacting protein 1 antibody
      • WRIP1_HUMAN antibody
      • WRNIP 1 antibody
      • WRNIP antibody
      • Wrnip1 antibody
      see all

    画像



    • Predicted band size : 72 kDa


      Western blot using ab4731 on HEK 293 lysate at 1/200.

      ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. The main band could be blocked using the immunising peptide.

    参考文献

    This product has been referenced in:
    • Mano Y  et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . Read more (PubMed: 17888034) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293)
    Loading amount
    10 µg
    Specification
    293
    Treatment
    lentivirus transduced for RNAi
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    投稿 Mar 05 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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