Anti-Werner's syndrome helicase WRN (phospho S1141) 抗体 (ab74158)

製品の概要

  • 製品名Anti-Werner's syndrome helicase WRN (phospho S1141) antibody
    Werner's syndrome helicase WRN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Werner's syndrome helicase WRN (phospho S1141)
  • 特異性ab74158 detects endogenous levels of Werner's syndrome helicase WRN only when phosphorylated at serine 1141.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide derived from human Werner's syndrome helicase WRN around the phosphorylation site of serine 1141 (S-S-SP-Q-P).

  • ポジティブ・コントロール
    • Extracts from K562 cells treated with etoposide (25uM, 24hours).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab74158 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 162 kDa.
ELISA 1/20000.

ターゲット情報

  • 機能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • 関連疾患Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 配列類似性Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻訳後修飾Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686C2056 antibody
    • DNA helicase antibody
    • DNA helicase, RecQ like type 3 antibody
    • Exonuclease WRN antibody
    • HGNC 12791 antibody
    • OTTHUMP00000225301 antibody
    • RecQ protein-like 2 antibody
    • RecQ-like type 3 antibody
    • RecQ3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • Werner syndrome ATP-dependent helicase antibody
    • Werner syndrome helicase antibody
    • Werner syndrome protein antibody
    • Werner syndrome, RecQ helicase like antibody
    • WRN antibody
    • WRN_HUMAN antibody
    see all

Anti-Werner's syndrome helicase WRN (phospho S1141) antibody 画像

  • All lanes : Anti-Werner's syndrome helicase WRN (phospho S1141) antibody (ab74158) at 1/500 dilution

    Lane 1 : Extracts from K562 cells treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from K562 cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 162 kDa
    Observed band size : 162 kDa

Anti-Werner's syndrome helicase WRN (phospho S1141) antibody (ab74158) 使用論文

ab74158 has not yet been referenced specifically in any publications.

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