Anti-Werner's syndrome helicase WRN 抗体 [EPR6392] (ab124673)

製品の概要

  • 製品名Anti-Werner's syndrome helicase WRN antibody [EPR6392]
    Werner's syndrome helicase WRN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to C-terminal amino acids of Human Werner's syndrome helicase WRN.

  • ポジティブ・コントロール
    • MOLT 4, K562 and A431 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
    • 関連疾患Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
      Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
    • 配列類似性Belongs to the helicase family. RecQ subfamily.
      Contains 1 3'-5' exonuclease domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.
    • 翻訳後修飾Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus > nucleolus. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp686C2056 antibody
      • DNA helicase antibody
      • DNA helicase, RecQ like type 3 antibody
      • Exonuclease WRN antibody
      • HGNC 12791 antibody
      • OTTHUMP00000225301 antibody
      • RecQ protein-like 2 antibody
      • RecQ-like type 3 antibody
      • RecQ3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • Werner syndrome ATP-dependent helicase antibody
      • Werner syndrome helicase antibody
      • Werner syndrome protein antibody
      • Werner syndrome, RecQ helicase like antibody
      • WRN antibody
      • WRN_HUMAN antibody
      see all

    Anti-Werner's syndrome helicase WRN antibody [EPR6392] 画像



    • Predicted band size : 162 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 µg)
      Lane 3: MOLT4 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 to 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
      ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 162 kDa

    Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) 使用論文

    ab124673 has not yet been referenced specifically in any publications.

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