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The epitope recognized by ab17987 maps to a region between residues 400 and 450 of human Werner Syndrome Helicase.
Our Abpromise guarantee covers the use of ab17987 in the following tested applications.
|WB||1/5000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
ab17987, at 0.1mcg/ml, staining Human WRN in whole cell lysate (100mcg) in 293T cells by Western blot. WRN was immunoprecipitated with ab17987 at 5mcg/10cm plate (+/- blocking peptide). Detection was by chemiluminescence with an exposure time of less than 10 seconds.