Anti-Werner's syndrome helicase WRN 抗体 (ab17987)
製品の概要
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製品名Anti-Werner's syndrome helicase WRN antibody
Werner's syndrome helicase WRN 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Werner's syndrome helicase WRN
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
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免疫原
The epitope recognized by ab17987 maps to a region between residues 400 and 450 of human Werner Syndrome Helicase.
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ポジティブ･コントロール
- Whole cell lysate from 293T cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab17987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/5000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
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関連疾患Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500]. -
配列類似性Belongs to the helicase family. RecQ subfamily.
Contains 1 3'-5' exonuclease domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻訳後修飾Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Nucleus > nucleolus. Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 7486 Human
- Omim: 604611 Human
- SwissProt: Q14191 Human
- Unigene: 632050 Human
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別名
- DKFZp686C2056 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 3 antibody
see all
画像
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Immunoprecipitation - Anti-Werner's syndrome helicase WRN antibody (ab17987)ab17987, at 0.1mcg/ml, staining Human WRN in whole cell lysate (100mcg) in 293T cells by Western blot. WRN was immunoprecipitated with ab17987 at 5mcg/10cm plate (+/- blocking peptide). Detection was by chemiluminescence with an exposure time of less than 10 seconds.
参考文献
This product has been referenced in:
- Otterlei M et al. Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest. J Cell Sci 119:5137-46 (2006). WB, IP ; Human . Read more (PubMed: 17118963) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"