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The epitope recognized by ab17987 maps to a region between residues 400 and 450 of human Werner Syndrome Helicase.
Our Abpromise guarantee covers the use of ab17987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
ab17987, at 0.1mcg/ml, staining Human WRN in whole cell lysate (100mcg) in 293T cells by Western blot. WRN was immunoprecipitated with ab17987 at 5mcg/10cm plate (+/- blocking peptide). Detection was by chemiluminescence with an exposure time of less than 10 seconds.