Anti-Werner's syndrome helicase WRN 抗体 (ab16489)

製品の概要

  • 製品名Anti-Werner's syndrome helicase WRN antibody
    Werner's syndrome helicase WRN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Werner's syndrome helicase WRN
  • 特異性ab16489 recognises a strong band of ~150kDa in mouse testis lysate corresponding to Werner's syndrome helicase WRN. A number of smaller, weakly cross-reacting bands are also seen. No bands were detected in either mouse ovary or mouse spleen lysate.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse Werner's syndrome helicase WRN.

  • ポジティブ・コントロール
    • mouse testes

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab16489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 162 kDa).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • 関連疾患Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 配列類似性Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻訳後修飾Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686C2056 antibody
    • DNA helicase antibody
    • DNA helicase, RecQ like type 3 antibody
    • Exonuclease WRN antibody
    • HGNC 12791 antibody
    • OTTHUMP00000225301 antibody
    • RecQ protein-like 2 antibody
    • RecQ-like type 3 antibody
    • RecQ3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • Werner syndrome ATP-dependent helicase antibody
    • Werner syndrome helicase antibody
    • Werner syndrome protein antibody
    • Werner syndrome, RecQ helicase like antibody
    • WRN antibody
    • WRN_HUMAN antibody
    see all

Anti-Werner's syndrome helicase WRN antibody 画像

  • All lanes : Anti-Werner's syndrome helicase WRN antibody (ab16489) at 1 µg/ml

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse testes lysate with Mouse Werner's syndrome helicase WRN peptide (ab19370) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

    Predicted band size : 162 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

Anti-Werner's syndrome helicase WRN antibody (ab16489) 使用論文

ab16489 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - other (in vitro expressed mice Werner 35S tagged)
Total protein in input 20 µg
Specification in vitro expressed mice Werner 35S tagged
Immuno-precipitation step Protein A
Username

Dr. amaury lachaud

Verified customer

投稿 Mar 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"