Anti-Werner's syndrome helicase WRN 抗体 [4H12] (ab66606)

製品の概要

  • 製品名
    Anti-Werner's syndrome helicase WRN antibody [4H12]
    Werner's syndrome helicase WRN 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4H12] to Werner's syndrome helicase WRN
  • アプリケーション
    適用あり: WB, IP, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human WRN helicase containing C-terminal hexahistidine produced in inscet Sf21 cells by baculovirus expression systems as described by Suzuki et al, and purified by affinity column chromatography using Ni-chelating NTA agarose gel.

  • エピトープ
    The epitope of 4H12 resides in the C-terminal 1373-1432 region.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    4H12
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 162 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • 関連疾患
    Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 配列類似性
    Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻訳後修飾
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus > nucleolus. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686C2056 antibody
    • DNA helicase antibody
    • DNA helicase, RecQ like type 3 antibody
    • Exonuclease WRN antibody
    • HGNC 12791 antibody
    • OTTHUMP00000225301 antibody
    • RecQ protein-like 2 antibody
    • RecQ-like type 3 antibody
    • RecQ3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • Werner syndrome ATP-dependent helicase antibody
    • Werner syndrome helicase antibody
    • Werner syndrome protein antibody
    • Werner syndrome, RecQ helicase like antibody
    • WRN antibody
    • WRN_HUMAN antibody
    see all

Anti-Werner's syndrome helicase WRN antibody [4H12] 画像

  • WRN helicase is illustrated above.The helicase domain is shown in black, and the nuclear localizing signal region is hatched. Epitope sites of 4H12 and 8H3 identified by the studies with various recombinant WRN helicases (Shiratori et. al., J. Cell Biol. 144: 1-9 (1999) are also shown.
  • All lanes : Anti-Werner's syndrome helicase WRN antibody [4H12] (ab66606) at 1/500 dilution

    Lane 1 : Extracts from cells of normal individuals
    Lane 2 : Extracts from cells of normal individuals
    Lane 3 : Extracts from cells of normal individuals
    Lane 4 : Extracts from cells of patients with Werner syndrome
    Lane 5 : Extracts from cells of patients with Werner syndrome
    Lane 6 : Purified recombinant WRN helicase made by baculovirus technology in the insect SF9 cells. See details published in the paper by Goto et al., Human Genet 105:301-307 (1999).


    Predicted band size : 162 kDa
  • Immunocytochemical staining of WRN helicase in HeLa cell by ab66606. See details published in the paper by Sakamoto et al., Genes to Cells 6: 421-430 (2001).

    a. WRN helicase in HeLa cells locating in the nucleoplasm and nucleolus before Camptothecin treatment.

    b. WRN helicase in HeLa cells forming muclear dots upon double-strand DNA break formation by Camptothecin treatment.
  • All lanes : Anti-Werner's syndrome helicase WRN antibody [4H12] (ab66606)

    Lane 1 : Werner syndrome patient
    Lane 2 : Normal individual
    Lane 3 : Normal individual


    Predicted band size : 162 kDa

Anti-Werner's syndrome helicase WRN antibody [4H12] (ab66606) 使用論文

This product has been referenced in:
  • Grierson PM  et al. BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription. Hum Mol Genet 21:1172-83 (2012). WB ; Human . Read more (PubMed: 22106380) »
  • Trego KS  et al. The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome. Cell Cycle 10:1998-2007 (2011). WB ; Human . Read more (PubMed: 21558802) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8-12%)
Sample
Human Cell lysate - whole cell (lymphocytes)
Specification
lymphocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
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Abcam user community

Verified customer

投稿 Jun 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
30 µg
Specification
HeLa
Treatment
siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa cells)
Loading amount
1000 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. april sandy

Verified customer

投稿 May 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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