Anti-WEE2 抗体 (ab121943)

製品の概要

  • 製品名Anti-WEE2 antibody
    WEE2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to WEE2
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: TKSGNHFEEP KLKDILLQIS LGLNYIHNSS MVHLDIKPSN IFICHKMQSE SSGVIEEVEN EADWFLSANV MYKIGDLGHA TSINKPKVEE GD, corresponding to amino acids 305-396 of Human WEE2.

  • ポジティブ・コントロール
    • A431 cells; Human duodenum tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/700. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
  • 組織特異性Expressed in testis.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation leads to increase its activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hCG_39453 antibody
    • WEE1 homolog 2 (S. pombe) antibody
    • Wee1-like protein kinase 1B antibody
    • Wee1-like protein kinase 2 antibody
    • WEE1B antibody
    • Wee1B kinase antibody
    • Wee2 antibody
    • WEE2_HUMAN antibody
    see all

Anti-WEE2 antibody 画像

  • ab121943, at 1/700 dilution, staining WEE2 in paraffin embedded Human duodenum tissue by Immunohistochemistry.
  • ab121943, at 4 µg/ml, staining WEE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Anti-WEE2 antibody (ab121943) 使用論文

ab121943 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (ovary)
Permeabilization Yes - 0.3% Triton X-100, 20 min
Specification ovary
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
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投稿 Mar 23 2015

Thank your for your inquiry.

The immunogen of ab121943 shows only 82% homology with mouse. We generally expect a cross reactivity from 85% homology. This is a border line case and I cannot recommend using this antibody in this case since WB i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"