製品の概要

  • 製品名
    Anti-Wee1 (phospho S53) antibody
    Wee1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Wee1 (phospho S53)
  • 由来種
    Rabbit
  • 特異性
    ab111820 detects endogenous levels of Wee1 only when phosphorylated at serine 53.
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from Human Wee1 around the phosphorylation site of Ser53 (E-D-SP-A-F).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab111820 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated during M and G1 phases. Also autophosphorylated.
    Ubiquitinated and degraded at the onset of G2/M phase.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686I18166 antibody
    • EC 2.7.10.2 antibody
    • FLJ16446 antibody
    • MGC105683 antibody
    • OTTHUMP00000231338 antibody
    • OTTHUMP00000231339 antibody
    • Wee 1 antibody
    • WEE 1 homolog 1 (S. pombe) antibody
    • WEE1 antibody
    • WEE1 homolog (S. pombe) antibody
    • Wee1 homolog antibody
    • WEE1 homolog S. pombe antibody
    • Wee1 like protein kinase antibody
    • Wee1 tyrosine kinase antibody
    • Wee1+ homolog antibody
    • Wee1+ S. pombe homolog antibody
    • WEE1, S. pombe, homolog of antibody
    • WEE1, somatic antibody
    • Wee1-like protein kinase antibody
    • WEE1_HUMAN antibody
    • WEE1A antibody
    • Wee1A kinase antibody
    • WEE1hu antibody
    see all

画像

  • ab111820 at 1/50 dilution staining Wee1 (phospho S53) in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized phosphopeptide.

プロトコール

参考文献

ab111820 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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