製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to WDR91
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 350 and 400 of human WDR91 (NP_054868.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate. 293T whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 配列類似性
    Contains 7 WD repeats.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSPC049 antibody
    • WD repeat containing protein 91 antibody
    • WD repeat domain 91 antibody
    • WD repeat-containing protein 91 antibody
    • WDR 91 antibody
    • WDR91 antibody
    • WDR91_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling WDR91 with ab80614 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-WDR91 antibody (ab80614) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 83 kDa
    Observed band size : 83 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab80614 at 3µg/mg lysate (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80614 at 1µg/ml and detected with chemiluminescence with an exposure time of 10 seconds.

参考文献

ab80614 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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