Anti-WDR91 抗体 (ab80614)
Key features and details
- Rabbit polyclonal to WDR91
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WDR91 antibody
WDR91 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR91 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residues 350 and 400 of human WDR91 (NP_054868.2).
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ポジティブ・コントロール
- HeLa whole cell lysate. 293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80614の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Contains 7 WD repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 29062 Human
- SwissProt: A4D1P6 Human
- Unigene: 459858 Human
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別名
- HSPC049 antibody
- WD repeat containing protein 91 antibody
- WD repeat domain 91 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling WDR91 with ab80614 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-WDR91 antibody (ab80614) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 30 seconds -
Immunoprecipitation of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab80614 at 3µg/mg lysate (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80614 at 1µg/ml and detected with chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab80614 は論文での使用が確認できていません。