製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to WDR79
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 1 and 50 of Human WDR79 (NP_060551.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage.
  • 組織特異性
    Expressed in all tissues and cell lines examined.
  • 関連疾患
    Defects in WRAP53 are the cause of dyskeratosis congenita autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
  • 配列類似性
    Contains 6 WD repeats.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus > Cajal body. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKCB3 antibody
    • FLJ10385 antibody
    • TCAB1 antibody
    • Telomerase Cajal body protein 1 antibody
    • WAP53_HUMAN antibody
    • WD repeat containing antisense to TP53 antibody
    • WD repeat containing protein 79 antibody
    • WD repeat-containing protein 79 antibody
    • WD40 repeat-containing protein encoding RNA antisense to p53 antibody
    • Wrap53 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling WDR79 with ab99376 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-WDR79 antibody (ab99376) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 59 kDa


    Exposure time : 30 seconds
  • Lane 1 : Detection of Human WDR79 by Immunoprecipitation from HeLa cells(1 mg for IP, 20% of IP loaded), using 3µg/mg lysate . Lane 2 : Control IgG

参考文献

This product has been referenced in:
  • Lee JH  et al. Involvement of SRSF11 in cell cycle-specific recruitment of telomerase to telomeres at nuclear speckles. Nucleic Acids Res 43:8435-51 (2015). IP, ICC/IF ; Human . Read more (PubMed: 26286192) »
  • Yuan P  et al. Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to telomeres and induces G1 cell cycle arrest in A549 cells. Oncol Lett 8:1009-1016 (2014). WB ; Human . Read more (PubMed: 25120649) »

See all 3 Publications for this product

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