製品の概要

  • 製品名Anti-WDR79 antibody
    WDR79 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to WDR79
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-548 of Human WDR79

  • ポジティブ・コントロール
    • WDR79 transfected mammalian cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage.
  • 組織特異性Expressed in all tissues and cell lines examined.
  • 関連疾患Defects in WRAP53 are the cause of dyskeratosis congenita autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
  • 配列類似性Contains 6 WD repeats.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > Cajal body. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ10385 antibody
    • TCAB1 antibody
    • Telomerase Cajal body protein 1 antibody
    • WAP53_HUMAN antibody
    • WD repeat containing protein 79 antibody
    • WD repeat-containing protein 79 antibody
    • WD40 repeat-containing protein encoding RNA antisense to p53 antibody
    • Wrap53 antibody
    see all

Anti-WDR79 antibody 画像

  • All lanes : Anti-WDR79 antibody (ab67994) at 1/500 dilution

    Lane 1 : WDR79 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 59 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-WDR79 antibody (ab67994) 使用論文

ab67994 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus tropicalis Cell lysate - whole cell (Oocyte)
Loading amount 4 cells
Specification Oocyte
Treatment Injected Tagged Construct and Un-injected
Gel Running Conditions Non-reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Eugene Gardner

Verified customer

投稿 Oct 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"