製品の概要

  • 製品名Anti-WDR5 antibody - ChIP Grade
    WDR5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to WDR5 - ChIP Grade
  • アプリケーション適用あり: WB, ChIP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ChIP Use at an assay dependent concentration. PubMed: 20864036
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
  • 配列類似性Belongs to the WD repeat WDR5/wds family.
    Contains 7 WD repeats.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
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Anti-WDR5 antibody - ChIP Grade 画像



  • Predicted band size : 36 kDa
  • Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-WDR5 antibody - ChIP Grade (ab56919) 使用論文

This product has been referenced in:
  • Ullius A  et al. The interaction of MYC with the trithorax protein ASH2L promotes gene transcription by regulating H3K27 modification. Nucleic Acids Res N/A:N/A (2014). Read more (PubMed: 24782528) »
  • Mungamuri SK  et al. Chromatin modifications sequentially enhance ErbB2 expression in ErbB2-positive breast cancers. Cell Rep 5:302-13 (2013). WB ; Human . Read more (PubMed: 24120871) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Aug 05 2015

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22512.

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