Anti-WDR5 抗体 [2C2] (ab56919)
Key features and details
- Mouse monoclonal [2C2] to WDR5
- Suitable for: WB, ICC/IF, ChIP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-WDR5 antibody [2C2]
WDR5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2C2] to WDR5 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, ChIP, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5
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特記事項
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4 -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
2C2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab56919の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration. PubMed: 20864036
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 20864036 |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. -
配列類似性
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 11091 Human
- Omim: 609012 Human
- SwissProt: P61964 Human
- Unigene: 397638 Human
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別名
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
画像
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WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/ml + Hs 181.Tes cell lysate
Predicted band size: 36 kDaThis image was generated using the ascites version of the product.
データシートおよび資料
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Datasheet download
参考文献 (33)
ab56919 は 33 報の論文で使用されています。
- Zhou T et al. Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Nat Commun 13:1855 (2022). PubMed: 35388006
- Zhang C et al. Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury. Cell Death Dis 13:770 (2022). PubMed: 36068197
- Yang B et al. Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly. J Virol 95:N/A (2021). PubMed: 33504601
- Wong CH et al. Ectopic HOTTIP expression induces noncanonical transactivation pathways to promote growth and invasiveness in pancreatic ductal adenocarcinoma. Cancer Lett 477:1-9 (2020). PubMed: 32120024
- Luo H et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice. Cancer Cell 36:645-659.e8 (2019). PubMed: 31786140