Anti-WDR33 抗体 (ab72115)
Key features and details
- Rabbit polyclonal to WDR33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-WDR33 antibody
WDR33 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR33 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to a region between residues 1286 and 1336 of human WDR33 (NP_060853.3).
-
ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72115の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
組織特異性
Most highly expressed in testis. -
配列類似性
Contains 1 collagen-like domain.
Contains 7 WD repeats. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 55339 Human
- SwissProt: Q6NUQ0 Human
- SwissProt: Q9C0J8 Human
- Unigene: 554831 Human
-
別名
- WD repeat containing protein 33 antibody
- WD repeat domain 33 antibody
- WD repeat-containing protein 33 antibody
see all
画像
-
All lanes : Anti-WDR33 antibody (ab72115) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 146 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of WDR33 by Western Blot of Immunprecipitate.
ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab72115 は論文での使用が確認できていません。